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X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E
a protein of interest is encoded downstream of the IPTG inducible promoter
Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate
This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers
or 2) the oxidation of ABA can occur to form phaseic acid and dihyhdrophaseic acid
HEPES free acid min.99.5% 103-88-8 X-Gluc is often used in
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